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reference plasmid pp5 vps36 759bp p3  (Bio-Rad)


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    Structured Review

    Bio-Rad reference plasmid pp5 vps36 759bp p3
    Reference Plasmid Pp5 Vps36 759bp P3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+plasmid+pp5+vps36+759bp+p3/Lyphochek+Urine+Metals+Control/pmc07583314-140-54-83
    Average 93 stars, based on 70 article reviews
    reference plasmid pp5 vps36 759bp p3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Functional Assay:

    Article Title: Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
    Article Snippet: .. Importantly, primers qP5-F1 and qP5-R1 can detect only the “standard” 5′ P -element end of 585 bp in length, but not the minimal functional 5′ P -element end of 140 bp in length that is present in enhancer-trap Gal4 transposons. qPCR was performed with 100 ng of genomic DNA or 5 pg of the reference plasmid pP5′-Vps36-759bp-P3′, 400 nM of each primer in a 25-μl reaction mixture using the HS-qPCR SYBR Blue Master Mix (Biolabmix), and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the following conditions: incubation at 95 °C for 5 min, followed by 39 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s. Data analysis was performed using CFX ManagerTM Software v3.0 (Bio-Rad). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
    Article Snippet: .. Importantly, primers qP5-F1 and qP5-R1 can detect only the “standard” 5′ P -element end of 585 bp in length, but not the minimal functional 5′ P -element end of 140 bp in length that is present in enhancer-trap Gal4 transposons. qPCR was performed with 100 ng of genomic DNA or 5 pg of the reference plasmid pP5′-Vps36-759bp-P3′, 400 nM of each primer in a 25-μl reaction mixture using the HS-qPCR SYBR Blue Master Mix (Biolabmix), and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the following conditions: incubation at 95 °C for 5 min, followed by 39 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s. Data analysis was performed using CFX ManagerTM Software v3.0 (Bio-Rad). ..

    Plasmid Preparation:

    Article Title: Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
    Article Snippet: .. Importantly, primers qP5-F1 and qP5-R1 can detect only the “standard” 5′ P -element end of 585 bp in length, but not the minimal functional 5′ P -element end of 140 bp in length that is present in enhancer-trap Gal4 transposons. qPCR was performed with 100 ng of genomic DNA or 5 pg of the reference plasmid pP5′-Vps36-759bp-P3′, 400 nM of each primer in a 25-μl reaction mixture using the HS-qPCR SYBR Blue Master Mix (Biolabmix), and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the following conditions: incubation at 95 °C for 5 min, followed by 39 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s. Data analysis was performed using CFX ManagerTM Software v3.0 (Bio-Rad). ..

    Incubation:

    Article Title: Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
    Article Snippet: .. Importantly, primers qP5-F1 and qP5-R1 can detect only the “standard” 5′ P -element end of 585 bp in length, but not the minimal functional 5′ P -element end of 140 bp in length that is present in enhancer-trap Gal4 transposons. qPCR was performed with 100 ng of genomic DNA or 5 pg of the reference plasmid pP5′-Vps36-759bp-P3′, 400 nM of each primer in a 25-μl reaction mixture using the HS-qPCR SYBR Blue Master Mix (Biolabmix), and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the following conditions: incubation at 95 °C for 5 min, followed by 39 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s. Data analysis was performed using CFX ManagerTM Software v3.0 (Bio-Rad). ..

    Software:

    Article Title: Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
    Article Snippet: .. Importantly, primers qP5-F1 and qP5-R1 can detect only the “standard” 5′ P -element end of 585 bp in length, but not the minimal functional 5′ P -element end of 140 bp in length that is present in enhancer-trap Gal4 transposons. qPCR was performed with 100 ng of genomic DNA or 5 pg of the reference plasmid pP5′-Vps36-759bp-P3′, 400 nM of each primer in a 25-μl reaction mixture using the HS-qPCR SYBR Blue Master Mix (Biolabmix), and the CFX96 Touch Real-Time PCR Detection System (Bio-Rad) under the following conditions: incubation at 95 °C for 5 min, followed by 39 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s. Data analysis was performed using CFX ManagerTM Software v3.0 (Bio-Rad). ..



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    reference plasmid pp5 vps36 759bp p3  (Bio-Rad)
    93
    Bio-Rad reference plasmid pp5 vps36 759bp p3
    Reference Plasmid Pp5 Vps36 759bp P3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+plasmid+pp5+vps36+759bp+p3/Lyphochek+Urine+Metals+Control/pmc07583314-140-54-83
    Average 93 stars, based on 1 article reviews
    reference plasmid pp5 vps36 759bp p3 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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